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plasmid free strain  (ATCC)


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    ATCC plasmid free strain
    Plasmid Free Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 20065 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+free+strain/Plasmid/pmc10100767-137-4-13
    Average 99 stars, based on 20065 article reviews
    plasmid free strain - by Bioz Stars, 2026-10
    99/100 stars

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    Plasmid Preparation:

    Article Title: Greater Invasion and Persistence of mcr-1- Bearing Plasmids in Escherichia coli than in Klebsiella pneumoniae
    Article Snippet: .. The culture of the plasmid-free strain ( E. coli C600 or K. pneumoniae ATCC 13883 RIF ) grown overnight was diluted at a 1:100 ratio into 2 mL fresh LB broth and mixed with 1:100,000 dilutions of mcr-1 plasmid-carrying strains ( E. coli C600/pHNGDE4P170, E. coli C600/pHNSHP45, K. pneumoniae ATCC 13883 RIF /pHNGDE4P170, or K. pneumoniae ATCC 13883 RIF /pHNSHP45) grown overnight. ..

    Article Title: Greater Invasion and Persistence of mcr-1- Bearing Plasmids in Escherichia coli than in Klebsiella pneumoniae.
    Article Snippet: .. The culture of the plasmid-free strain (E. coli C600 or K. pneumoniae ATCC 13883RIF) grown overnight was diluted at a 1:100 ratio into 2 mL fresh LB broth and mixed with 1:100,000 dilutions of mcr-1 plasmid-carrying strains (E. coli C600/pHNGDE4P170, E. coli C600/pHNSHP45, K. pneumoniae ATCC 13883RIF/pHNGDE4P170, or K. pneumoniae ATCC 13883RIF/pHNSHP45) grown overnight. ..



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    All plasmids generated in this study

    Journal: Microbial Cell Factories

    Article Title: Scalable protein production by Komagataella phaffii enabled by ARS plasmids and carbon source-based selection

    doi: 10.1186/s12934-024-02368-3

    Figure Lengend Snippet: All plasmids generated in this study

    Article Snippet: It was kindly provided by Bisy GmbH (Hofstaetten a. d. Raab, Austria) and was made by deletion of the GUT1 gene of the killer plasmid-free Mut S platform strain K. phaffii BG11 (Biogrammatics, Carlsbad, CA).

    Techniques: Generated, Selection, Marker, Clone Assay, Expressing

    Vector map of a general ARS plasmid carrying a carbon marker cassette. Besides an origin of replication and an Ampicillin resistance cassette for cloning in E. coli , the episomal plasmids used in this study harbor the PARS1 sequence for the replication in K. phaffii , a reporter cassette with the de-repressable P DC promoter driving Ca lB expression, whose secretion is facilitated by the D-alpha signal sequence, a deletion variant of the S. cerevisiae mating factor alpha signal peptide. Selective pressure in K. phaffii is provided by either GUT1 or TPI1 genes with one of the 5 tested promoters placed upstream

    Journal: Microbial Cell Factories

    Article Title: Scalable protein production by Komagataella phaffii enabled by ARS plasmids and carbon source-based selection

    doi: 10.1186/s12934-024-02368-3

    Figure Lengend Snippet: Vector map of a general ARS plasmid carrying a carbon marker cassette. Besides an origin of replication and an Ampicillin resistance cassette for cloning in E. coli , the episomal plasmids used in this study harbor the PARS1 sequence for the replication in K. phaffii , a reporter cassette with the de-repressable P DC promoter driving Ca lB expression, whose secretion is facilitated by the D-alpha signal sequence, a deletion variant of the S. cerevisiae mating factor alpha signal peptide. Selective pressure in K. phaffii is provided by either GUT1 or TPI1 genes with one of the 5 tested promoters placed upstream

    Article Snippet: It was kindly provided by Bisy GmbH (Hofstaetten a. d. Raab, Austria) and was made by deletion of the GUT1 gene of the killer plasmid-free Mut S platform strain K. phaffii BG11 (Biogrammatics, Carlsbad, CA).

    Techniques: Plasmid Preparation, Marker, Clone Assay, Sequencing, Expressing, Variant Assay